Figure 5

Negative immunoreactivity for PLCβ2 and α-gustducin in the TRPM5-expressing microvillous cells. A. An image of GFP-expressing microvillous cells (asterisks) and OSNs. B. The antibody against the PLCβ2 labeled OSNs in a pattern of the membrane of OSNs and some apical regions pointed by arrows, which might be apical microvilli of a different population microvillous cells reported previously[26]. C. Overlaid image of A and B showing the antibody did not label the TRPM5-expressing microvillous cells. Inset is an image from a control experiment, in which the PLCβ2 antibody was pre-absorbed to the specific antigen peptide, showing no immunoreactivity. D. The antibody against α-gustducin did not label the TRPM5-expressing microvillous cells in the OE, although the antibody at the same condition labeled nicely the TRPM5-expressing solitary chemosensory cells in the respiratory epithelium (inset). E. Image of the TRPM5-expressing microvillous cells from the same OE section overlaid with the image of D, showing there was no α-gustducin immunoreactivity in these microvillous cells. F. The image of E was overlaid with the bright field image. Note the apical microvilli of these cells reach to the lumen. Scales: 20 μm; inset, 10 μm.