Figure 3

GFP positive microvillous cells from mouse olfactory epithelium have voltage-gated Na⁺ currents and a Ca²⁺-activated cation current. A. Isolated cells from mouse olfactory epithelium used for patch clamp recording were identified based on GFP fluorescence and the presence of a microvillous structure. Scale bar is 10 μm. B. Currents in response to voltage steps from -80 mV to from -50-0 mV, in 10 mV increments. Dotted line indicates the zero current level. Note the appearance of a fast voltage-activated Na⁺ current in this cells. C. Peak inward current as a function of voltage from the experiment in (A). D. Current in response to UV uncaging of DMNP-EDTA (caged Ca²⁺). Note the large increase in the outward current (measured at +80 mV; open symbol) and absence of response at -80 mV (filled symbol). E. Currents in response to ramp depolarization at time (a) and (b) from the experiment shown in D. Data are representative of three independent experiments.