Figure 2

Immunoreactivity of TRPM5 in GFP-expressing microvillous cells. A. A confocal image showing that positive immunoreaction for TRPM5 (red) was present in cells located superficially. B. The image of GFP was overlaid onto A, showing co-localization of TRPM5 immunoreactivity and GFP expression. The images were acquired from a section of medial surface of the endo-turbinate where there were no TRPM5-expressing ciliated OSNs. C. A magnified image showing the TRPM5 immunoreactivity (red) in GFP expressing cells. D. Negative control. The antibody did not label GFP expressing cells in the olfactory epithelia of TRPM5-KO-GFP mice. Scales: 20 μm.