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Figure 8 | BMC Neuroscience

Figure 8

From: The Kv2.1 K+ channel targets to the axon initial segment of hippocampal and cortical neurons in culture and in situ

Figure 8

Localization of Kv2.1 clusters to the AIS of layer IV cortical neurons in situ. Rat brain formaldehyde-fixed cyrosections obtained from post natal day 21 animals were immuno-stained with polyclonal antibody against Kv2.1 and monoclonal antibody against ankyrin G. The anti-Kv2.1 antibody was detected with Alexa 488-conjugated goat anti-rabbit secondary antibody (green) while the anti-AnkG monoclonal antibody was detected with Alexa 594-conjugated goat anti-mouse secondary antibody (red). Single optical sections of either Kv2.1 or ankyrinG immuno-staining are shown in panels A and B. Panel C shows the overlay of these two images and panel D contains a magnification of the boxed region in C. Panels E and F show a maximum projection image (Z-stack compression) corresponding to the areas shown in panels C and D. The arrows denote the expression of Kv2.1 within AnkG-positive AIS domains (arrows).

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