Figure 8From: The Kv2.1 K+ channel targets to the axon initial segment of hippocampal and cortical neurons in culture and in situLocalization of Kv2.1 clusters to the AIS of layer IV cortical neurons in situ. Rat brain formaldehyde-fixed cyrosections obtained from post natal day 21 animals were immuno-stained with polyclonal antibody against Kv2.1 and monoclonal antibody against ankyrin G. The anti-Kv2.1 antibody was detected with Alexa 488-conjugated goat anti-rabbit secondary antibody (green) while the anti-AnkG monoclonal antibody was detected with Alexa 594-conjugated goat anti-mouse secondary antibody (red). Single optical sections of either Kv2.1 or ankyrinG immuno-staining are shown in panels A and B. Panel C shows the overlay of these two images and panel D contains a magnification of the boxed region in C. Panels E and F show a maximum projection image (Z-stack compression) corresponding to the areas shown in panels C and D. The arrows denote the expression of Kv2.1 within AnkG-positive AIS domains (arrows).Back to article page