Change in BOLD signal over time. Shown are representative time course data from select brain areas depicting the change in BOLD signal following stimulus presentation (time 0 min). For each brain area the data in response to mate alone or mate/intruder are presented on the left column while the data from mate/intruder (aggression) or novel receptive female (sex) in the presence of SRX251 or fluoxetine are presented in the middle and right columns. The dashed horizontal line marks the threshold of 2% below which is baseline noise in awake imaging studies. The solid line at top represents the approximate BOLD signal change for aggressive motivation alone. The scale marked piloerection shows the range of time (62 ± 11) for piloerection following introduction of the mate/intruder into the vivarium. The percentage change in BOLD signal intensity at each time point (100 data acquisition over the 10 min scanning period) is the average of 10 male residents for each experimental condition. Vertical lines at each data point denote the standard error of the mean. Where show, the F values represent significant differences between experimental conditions based on a repeated measured 2-way ANOVA for the 1st min post stimulation.