Expression of Galpha14 in sweet-transducing taste cells of the posterior tongue

BMC Neuroscience

Table 2 Primers for RT-PCR

Protein & Gene		Accession #	Forward Primer (5'→3')	Reverse Primer (5'→3')	Product bp	Anneal °C
Gα14	Gna14	NM_008137	attagctacttcccagagtacaca	gctcagatcaccctctgtct	256	62°C
			* tcatgcaacagagggacttg	* agggccatgctcaattacac	294	60°C
Gαq	Gnaq	NM_008139	gtcgactacttcccagaatatgat	agtccaggacggcaataaat	333	62°C
			* aacacacaccatccgtcaga	* ggcaagcagtggtctctagc	229	60°C
Gα11	Gna11	NM_010301	agcccaagtcctgagtttga	tgccaagtcagagtggagaa	236	60°C
Gαgus	Gnat3	NM_001081143	gcaaccacctccattgttct	agaagagcccacagtctttgag	286	58°C
PLCβ2	Plcb2	NM_177568	gagcaaatcgccaagatgat	ccttgtctgtggtgaccttg	163	60°C
SNAP25	Snap25	NM_011428	ggcaataatcaggatggagtag	agatttaaccacttcccagca	310	58°C
T1R2	Tas1r2	NM_031873	aagcatcgcctcctactcc	ggctggcaactcttagaacac	114	58°C
T1R3	Tas1r3	NM_031872	gaagcatccagatgacttca	gggaacagaaggacactgag	283	58°C
TrpM5	Trpm5	NM_020277	gtctggaatcacaggccaac	gttgatgtgccccaaaaact	234	58°C
β-actin	Actb	NM_007393	ccctgtgctgctcacc	gcacgatttccctctcag	328	58°C

Primers marked * (Gα14 and Gαq) are located closer to the mRNA 3' end and were only used on amplified RNA from pools of GFP-expressing cells (Fig. 2).

ISSN: 1471-2202