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Table 1 T1R & G-protein co-localization in Taste Buds

From: Expression of Galpha14 in sweet-transducing taste cells of the posterior tongue

  Immunocytochem.   Single Cell RT-PCR
TrpM5 with   % TrpM5 with   %
Gq/11/14 14/27 51.9 Gα14 13/21 61.9
Gαgus 17/27 63.0 Gαgus 15/21 71.4
Gq/11/14 + Gαgus 4/27 14.8 Gα14 + Gαgus 7/21 33.3
Gq/11/14 with    Gα14 with   
T1R3(GFP) 30/35 85.7 T1R3 13/14 92.9
    T1R2 10/14 71.4
    T1R3 + T1R2 10/14 71.4
T1R3(GFP)    T1R3+T1R2 with   
(includes T1R1 & TT1R2)    Gαq 5/10 50.0
Gq/11/14 30/41 73.1 Gα14 10/10 100.0
Gαgus 11/41 26.8 Gαgus 4/10 40.0
  1. Results from immunocytochemistry are not significantly different (p > 0.05) from data from single cell RT-PCR (Fisher's Exact Test). The immunocytochemical data and RT-PCR data showing co-expression of gustducin with T1Rs is not directly comparable since some cells express only T1R3 and not T1R2+T1R3