Gq/11/14 immunoreactivity in Type II cells. (A) Co-localization of Gq/11/14 (red), Gαgus (blue) in the vallate papilla of a TrpM5-GFP (green) animal. TrpM5 is a marker of Type II taste cells. Gq/11/14 stains about half (14 of 27) of the TrpM5-GFP cells. Usually the Gαq-family immunoreactive cells are different from those positive for Gαgus (blue). A', A", represent single staining of TrpM5-GFP TBs used to obtain the merged pictures A. The figure in Additional file 1 shows single plane confocal images from this image set for comparison. (B) PGP9.5 (blue), a marker of Type III cells, does not co-localize with either Gq/11/14 (red) or TrpM5 (green). The figure in Additional file 2 shows single plane confocal images from this image set for comparison. Scale bar 20 μM.