Figure 2
From: Expression of Galpha14 in sweet-transducing taste cells of the posterior tongue
Gα14 is expressed only in Type II/Receptor cells. Three pools (#1, 2, 3), each containing ten individual GFP(+) taste cells were collected from PLCβ2-GFP mice (plc pools) or from GAD-GFP mice (gad pools). Amplified mRNAs from these pools represent Type II and Type III cells respectively and were analyzed by RT-PCR. RNA from a cluster of non-taste epithelial cells (NT) and a taste bud (TB) were amplified and analyzed in parallel. A negative control (-) reaction was run with no cDNA. RT-PCR for PLCβ2 (Type II cells), T1R3 (Type II cells) and SNAP25 (Type III cells) [29] confirmed that the pools were not cross-contaminated with cells of the opposing type.