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Figure 1 | BMC Neuroscience

Figure 1

From: Long-term neprilysin gene transfer is associated with reduced levels of intracellular Abeta and behavioral improvement in APP transgenic mice

Figure 1

Immunocytochemical analysis of the levels of NEP expression in brains of APP tg mice that received lentiviral injection. Panels A-F represent images obtained by LSCM with sections from APP tg mice (12 m/o) injected into the hippocampus and neocortex. (A) Baseline levels of endogenous NEP expression. (B) Increased expression of NEP in the neocortex and hippocampus of mice injected with LV-NEP. (C) Animals that received injections with LV-NEPX expressed levels of NEP comparable to animals injected with LV-NEP. (D-F) Higher magnification views of neurons in the neocortex showing the increased expression of NEP in LV-NEP injected animals. (G-I) Double-labelling confocal analysis demonstrating colocalization of NEP (red channel) in neurons expressing human APP (green channel) in the APP tg mice injected with LV-NEP. Bar is (A-C) 200 μm, (D-F) 10 μm, (G-I) 7 μm. (J-L) Western blot and image analysis of levels of NEP and APP/Aβ (6E10 and CT15) immunoreactivity in APP tg mice treated with lentiviruses. (M) Levels of NEP activity in the cortex of APP tg mice treated with lentiviruses. *p < 0.05 compared to LV-empty by one-way ANOVA with post-hoc Dunnett's, n = 8 mice per group

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