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Table 1 Relative percentage of VGLUT1 and VGAT mRNA levels compared to β-actin expression.

From: The role of the t-SNARE SNAP-25 in action potential-dependent calcium signaling and expression in GABAergic and glutamatergic neurons

Fluorescence-activated cell sorting
Sample GFP Phenotype VGLUT1 VGAT
Immature cortical cells Negative 2.37% n. d.*
  Positive n. d. 7.89%
Adult cortical cells Negative 3.91% n. d.
  Positive n. d. 9.51%
Laser capture microdissection
Region GFP Phenotype VGLUT1 VGAT
Reticular Nucleus Positive n. d. 12.68%
Caudate Positive n. d. 14.07%
Purkinje cells Positive n. d. 15.39%
Hippocampal interneurons (CA1) Positive n. d. 12.45%
Hippocampal pyramidal cells (CA1) Negative 11.95% n. d.
Cerebellar granule cells Negative 13.56% n. d.
  1. * n. d. (not detected) is considered less than 0.001% (i.e. Ct value > 10 cycles) of gene expression in comparable amounts of whole brain samples (see Materials and Methods).