Figure 3

Fos immunoreactivity: Fos-immunostained cells in coronal section of A/ NTS, B/ PVN, C/ DMH, D/ Number of Fos-immunopositive cells in NTS, PVN and DMH 60 minutes after i.c.v. application of saline (SAL), CCK-8 (4 μg/kg, i.p.), CART(61-102) (0.1 μg/mouse, i.c.v.), and their combination expressed unilaterally per animal (n = 4–5) and section (n = 5–6). ⁺ P < 0.01 vs. SAL, CCK and CART, * P < 0.05, ** P < 0.01 vs. SAL [ANOVA, for NTS F_3,16 = 26.23, for PVN F_3,13 = 100.35, for DMH F_3,12 = 58.19]. SAL – saline, NTS – solitary tract nucleus, CC – central canal, AP – area postrema, PVN – paraventricular nucleus. Bar = 100 μm.