Tau phosphorylation is increased by anaesthesia but remains partially sensitive to insulin even during hypothermia. Samples described in Fig. 2 and Fig. 3 were analysed for phosphorylation of tau (A-representative blots) at Ser202 and Thr205 (AT8 antibody), and for total tau (tau-5 antibody). The results from each treatment group were quantified by densitometry (B). The mean ± SD, relative to the control animals, is shown. Significant differences between control and each treatment group are given, *p < 0.05, **p < 0.005.