| Bud diameter (μm) | Section thickness (μm) | Number of GFP cells | Number of PLCβ2 cells | Number of SNAP25 cells |
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Fungiform taste buds | 40.1 ± 4.2 (n = 18) | 12.8 ± 5.6 (n = 40) | 2.8 ± 1.3 (n = 40) | 4.2 ± 1.9 (n = 20) | 1.6 ± 1.1 (n = 20) |
Circumvallate taste buds | 43.3 ± 7.4 (n = 29) | 14.2 ± 6.0 (n = 38) | 7.5 ± 2.7 (n = 42) | 6.0 ± 1.9 (n = 20) | 4.4 ± 1.8 (n = 22) |
- The diameter of the taste buds was similar in fungiform and circumvallate taste buds, indicating similar numbers of cells in each type of taste bud. However, the number of GFP-positive cells, SNAP-25-ir and PLCβ2-ir cells was significantly higher in the circumvallate taste buds than in the fungiform taste buds (Student's t test; p < 0.01; df = 80 for GFP, df = 40 for SNAP-25 and df = 38 for PLCβ2). Note that in the fungiform taste buds, the PLCβ2-ir cells are significantly more numerous than the TRPM5-GFP cells (Student's t test; p < 0.01; df = 19).