Expression of TRPM5-GFP and SNAP-25 in confocal images of fungiform and circumvallate taste buds. The top figures illustrate the expression of GFP under the control of the TRPM5 promoter (green) and SNAP-25-ir (red) in fungiform taste buds and the bottom figures illustrate labeling in circumvallate taste buds. No co-localization was observed between TRPM5-GFP and SNAP-25-ir. The number of SNAP-25-ir and GFP-labeled cells was larger in circumvallate than in fungiform taste buds. Each figure represents merged images from a Z-series. Scale bar: 10 μm.