Expression of TRPM5-GFP and PLCβ2 in confocal images of fungiform and circumvallate taste buds. The top figures illustrate the expression of GFP under the control of the TRPM5 promoter (green) and PLCβ2-ir (red) in fungiform taste buds. The bottom figures illustrate labeling in circumvallate taste buds. Note that in circumvallate taste buds there are far more TRPM5-GFP cells relative to fungiform taste buds, however PLCβ2 and TRPM5-GFP were generally co-localized in both fungiform and circumvallate taste buds. Each figure represents merged images from a Z-series. Scale bar: 10 μm.