Figure 6

Squalestatin reduces the association between MoPrP105-132 and cPLA ₂ and COX-1. Neuroblastoma cells were pre-treated with either 1 μM squalestatin or 2 μM Hexa-PAF prior to incubation with 30 μM MoPrP105-132-biotin for 5 minutes at 37°C, fixed and stained with Texas Red-streptavidin (red) anti-cPLA₂-FITC or anti-COX-1-FITC (green). Nuclei were revealed using Vectashield with DAPI (blue). Images show the extent of co-localisation (yellow) between MoPrP105-132 (red) and cPLA₂ (green) (A) or between MoPrP105-132 (red) and COX-1 (green) (B) in squalestatin-treated cells. Similarly, images show the extent of co-localisation (yellow) between MoPrP105-132 (red) and cPLA₂ (green) (C) or between MoPrP105-132 (red) and COX-1 (green) (D) in Hexa-PAF-treated cells. Scale bars, 5 μm.