Figure 3

Squalestatin reduces the distribution of MoPrP105-132 and CTxB in neuroblastoma cells. Neuroblastoma cells were incubated with 1 μM squalestatin for 24 hours and subsequently incubated with MoPrP105-132-rhodamine (red) and CTxB-Alexa Fluor 488 (green), nuclei were revealed using Vectashield with DAPI (blue). (A) Lack of co-localisation was apparent between MoPrP105-132 and CTxB in squalestatin treated cells compared with (B) untreated cells. Scale bar, 5 μm. (C) Isolation of lipid raft/non-raft membranes from neuroblastoma cells treated with 1 μM squalestatin for 24 hours. In squalestatin-treated cells the majority of MoPrP105-132 was present in the non-raft fraction.