Figure 4

Co-localisation Grik1/GluR5 mRNA with markers of defined sub-populations of sensory neurons: Double in situ hybridization was carried out on sections of adult DRG by using fluorescein/fast red detection for TrkA (A), TrkB (D), TrkC (G), c-ret (J) and immunohistochemical detection of isolectin B4 (M). Grik1/GluR5 mRNA was subsequently detected using DIG/NBT-BCIP detection in B, E, H, K, N. In situ signals were converted into pseudo colors and images were superimposed (C, F, I, L, O) to show co-labelling of cells. Grik1/GluR5 was excluded from TrkA, B and C population but was co-localised with c-ret and isolectin B4 neurons. Scale bar 50 μm.