Table 2 Grading of neurological severity score of rats.

Hemiplegia: inability resist to forced changes in position

1

Flexion in hindlimb when raised by tail

1

Inability to walk straight when placed on floor

1

Inability to walk

1

Reflex

Loss of righting reflex for

20 minutes

1

40 minutes

1

60 minutes

1

Limb reflexes: loss of placing Reflexes

Left forelimb

1

Left hindlimb

1

Right forelimb

1

Right hindlimb

1

Clinical grade

Loss of seeking behavior

1

Prostration

1

Inability to exit from a circle (50 cm in diameter) when left in the center for Functioning test: failure in beam balancing task (1 cm wide)

20 minutes

1

40 minutes

1

60 minutes

1

Balances with steady posture, paws on top of beam

1

Grasps sides of beam and/or has shaky movement

1

One or more paws slip off beam

1

Attempt to balance on beam but falls off

1

Drapes over beam and/or hangs on beam and falls off

1

Falls off beam with no attempt to balance or hang on

1

Failure in beam walking task

2.5-cm wide

1

5.0-cm wide

1

8.0-cm wide

1

Sum

25