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Table 1 Previous studies employing microarray approaches to study stroke

From: A microarray study of gene and protein regulation in human and rat brain following middle cerebral artery occlusion

  Soriano et al. 2000 Jin et al. 2001 Kim et al. 2002 Rao et al. 2002 Schmidt-Kastner et al. 2002 Tang et al. 2002 Roth et al. 2003 Kim et al. 2004 Lu et al. 2004 Moore et al. 2005 Ford et al. 2006 Tang et al. 2006 Vikman and Edvinsson 2006 Our data
Material used Rat brain tissue Rat brain tissue Rat brain tissue Rat brain tissue Rat brain tissue Rat brain tissue Rat brain tissue Rat brain tissue Rat brain tissue   Rat brain tissue    
Model of ischemia Permanent focal MCAO Transient global MCAO Permanent focal MCAO Transient focal MCAO Transient focal MCAO Permanent focal MCAO Permanent focal MCAO Transient focal MCAO Transient focal MCAO Blood from ischemic stroke patients Permanent and transient focal MCAO Blood from ischemic stroke patients Post-mortem brain tissue from 11 stroke patients Post-mortem brain tissue from 12 stroke patients and permanent focal rat MCAO
No of genes 750 374 1176 1263 9044 ~8,000 ~13,000 5,000 1,322 ~19,000 8784 ~39,000 7458 1176
Time after ischemia 3 hours 4 hours
24 hours
72 hours
6 hours 6 hours
24 hours
5 hours 24 hours 1 hours
3 hours
6 hours
24 hours
3 hours
6 hours
12 hours
1 days
2 days
4 days
30 min
4 hours
8 hours
24 hours
3 days
7 days
As soon as possible after hospitalization 24 hours 3 hours
5 hours
24 hours
7–10 days (obtained 2–3 days post-mortem) 1 hour-21 days (rat) and 2–37 days (human, obtained by 6 hours post-mortem)
Cut-off values 2.0-fold 1.7-fold 2.0-fold 2.5-fold 1.7-fold 2.0-fold 3.0-fold 2.0-fold 2.0-fold - 2.0-fold 1.5-fold - 2.0-fold
Confirmation of results In situ hybridization, western blotting Western blotting, immuno-histochemistry RT-PCR Real-time PCR, antisense knockdown, western blotting, immuno-histochemistry Microarray analysis only Real-time RT-PCR Cell culture, in situ hybridization, western blotting, immuno-fluorescence Cell culture, northern blotting, RT-PCR, western blotting, immuno-histochemistry Real-time RT-PCR Real-time RT-PCR Microarray analysis only Microarray analysis only Real-time PCR, immuno-histochemistry Cell culture, RT-PCR, western blotting, immuno-histochemistry, immuno-fluorescence
Selected molecules NGFI-C
ARC
GRB2
SMN1
IFN-IP
NDGAP-1
NPR
SOCS-3   NARP
SPR
SPIN2C
ARG1
LBP
PC4 FAK Synaptic proteins CD14
CD36
FcGR2A
IFNGR1 caspase-1 a-catenin
   LY64
ELK3
POU3F4
RHOA
PAK1
MMP11
INI1