12 μm sagittal brain sections of a two-month-old rat were processed for immunohistochemistry. The top sections (A, B, C) show white matter and granula within a cerebellar fold (average projection of 15 z-stacks of 0.16 micron each). The DAPI stained nuclei appear in blue and reveal the nuclei-dense granula regions bracketing the white matter. The bottom sections (D, E, F) show the ventral reticular nucleus (average projection of 9 z-stacks of 0.16 micron each). The sections were incubated with anti-Necl-3 and anti neurofilament antibodies, which were revealed with secondary antibodies coupled to Alexa 488 and Alexa 594, respectively. Panels (A) and (D) show neurofilament immunoreactivity in the green-pass channel, and panels (B) and (E) Necl-3 immunoreactivity in the red-pass channel. The green and red images are merged in (C) and (F). Nuclei were visualized with DAPI in (A) and (B). The inset of Figure 5F shows that Necl-3 and neurofilament do not perfectly overlap.