Figure 15

(A) The image in the red-pass channel shows Fc-Necl-3 revealed by a rhodamine-coupled secondary antibody. (B) The green-pass channel shows that Fc-Necl-3 binds to oligodendrocytes, which are identified by the specific mAb RIP revealed with an Alexa 594 secondary antibody. Note that Rip-negative cell-types are not bound by Fc-Necl-3 (arrows in A and B). The green and red images are merged in (C). Nuclei were visualized with DAPI in (A), (B). The field shown in the figure is representative of more than 100 rhodamine-positive cells, all of which were also Rip-positive.