Figure 13

12 μm trigeminal nerve sections of a four-month-old rat were processed for immunohistochemistry. The sections were incubated with anti-Necl-3 and anti neurofilament antibodies (A, B, C, 10 z-stacks of 0.24 micron each) or with anti-Necl-3 and anti MBP antibodies (D, E, F, 10 z-stacks of 0.12 micron each), which were revealed with secondary antibodies as in Fig. 5. (A), neurofilament immunoreactivity in the green-pass channel; (B), Necl-3 immunoreactivity in the red-pass channel; panel (D), Rip immunoreactivity in the green-pass channel; (E), Necl-3 immunoreactivity in the red-pass channel. The green and red images are merged in (C) and (F). Nuclei were visualized with DAPI in (A), (B), (D), and (E).