12 μm sagittal sections of the caudate putamen in a two-month-old rat were processed for immunohistochemistry. The sections were incubated with anti-Necl-3 and anti Rip antibodies, which were revealed with secondary antibodies as in Fig. 5. (A), Rip immunoreactivity in the green-pass channel; (B), Necl-3 immunoreactivity in the red-pass channel. The green and red images are merged in (C). Nuclei were visualized with DAPI in (A) and (B). All images represent the average projection of 9 z-stacks of 0.12 micron each. Note that although present in the same areas, the Necl-3 and oligodendrocytic immunoreactivities are not overlapping.