12 μm sagittal brain sections of a two-month-old rat were processed for immunohistochemistry. The vestibular nucleus is visualized in the top sections (A, B, C) as the average projection of 20 z-stacks of 0.24 micron each, and in the bottom sections the pons (D, E, F) as the average projection of 26 z-stacks of 0.12 micron each. The sections were incubated with anti-Necl-3 and anti Rip antibodies (A, B, C) or with anti-Necl-3 and anti MBP antibodies (D, E, F), which were revealed with secondary antibodies as in Fig. 5. (A), Rip immunoreactivity in the green-pass channel; (B), Necl-3 immunoreactivity in the red-pass channel; (D), MBP immunoreactivity in the green-pass channel; (E), Necl-3 immunoreactivity in the red-pass channel. The green and red images are merged in (C) and (F). Nuclei were visualized with DAPI in (A), (B), (D), and (E).