12 μm sagittal brain sections of a two-month-old rat were processed for immunohistochemistry. The sections were incubated with anti-Necl-3 antibody, which was revealed with a secondary antibody coupled to Alexa 594. Nuclei were visualized with DAPI. Panel A (average projection of 64 z-stacks of 0.12 micron each) shows part of the third ventricle (asterisk) bordered by the ependymal cell layer. Panel B (average projection of 57 z-stacks of 0.12 micron each) shows ependymal cells of the choroid plexus in the third ventricle.