Figure 3
From: Amyloid-like aggregates of neuronal tau induced by formaldehyde promote apoptosis of neuronal cells
Changes in fluorescence spectra of thioflavin T and absorption spectra of Congo Red in the presence of tau deposits. (A) Thioflavin T (10 μM final concentration) was incubated with 0.1% aldehyde-treated tau (2 μM) in 100 mM potassium phosphate buffer pH 7.2 for 15 min before measurement. Emission spectra of ThT were recorded (excitation at 450 nm) in the presence of formaldehyde-treated tau (curve 1), self-aggregated tau (curve 2), acetaldehyde-treated tau (curve 3) and in the absence of protein (curve 4). (B) Kinetics of the increase in the fluorescence emission of ThT incubated with formaldehyde-treated tau (curve 1) or self-aggregated tau (curve 2). (C) Under the same conditions, Congo Red (5 μM final concentration) was incubated with formaldehyde-treated tau (curve 1), acetaldehyde-treated tau (curve 2) or native tau (curve 3) for 15 min before measurement. Congo Red alone is shown as a control (curve 4).