Figure 1
From: Amyloid-like aggregates of neuronal tau induced by formaldehyde promote apoptosis of neuronal cells
Effect of formaldehyde on tau aggregation at different concentrations. (A) Recombinant htau-40 (20 μM final concentration) was incubated with formaldehyde at desired concentrations in 100 mM phosphate buffer (pH 7.2) at 37°C for 24 h and aliquots (10 μl) were loaded for 10% SDS-PAGE. Lane M contains molecular mass standards. (B) BSA was used as a control. (C) Gray densities of tau polymers on SDS-PAGE were measured (curve 1) and changes in the light scattering of tau with formaldehyde at different concentrations were detected (curve 2). (D) The gray densities of BSA monomers from panel B (curve 1) and the light scattering of BSA (curve 2). (E) Tau-40 (1.2 μM final concentration) was incubated with 0.1 % formaldehyde and changes in the light scattering at 480 nm were measured at different time intervals in the presence (curve 1) or absence (curve 2) of formaldehyde. BSA alone (curve 3) or BSA incubated with formaldehyde (curve 4) was used as controls. (F) The same data as shown in panel E is plotted on a semi-logarithmic scale [36].