Western blot analysis of forebrain selective neuronal β3 knockouts. Western blot analysis of the β3 subunit of the GABAA-R from individual 13–16 week old mice revealed dramatically reduced amounts of β3 in hippocampus (A) and cortex (B) of α CamKII-cre positive samples compared to cre negative control samples. The amount of β3 in cerebellum (C) did not differ between genotypes. Analysis of cortex for the GABAA-R α1 subunit revealed a ~95% reduction in knockout samples compared to cre negative controls. All blots were reprobed for β-actin as a loading control. Shown are representative western blots. Four mice of each genotype were analyzed and all samples were analyzed on at least two different blots. D. Summary graph of western blot analysis demonstrating a significant (*, p ≤ 0.01; **, p < 0.001) reduction in β3 protein in hippocampus and cortex, but not in cerebellum. Data are expressed as mean ± SEM of percent change in band intensity relative to cre negative controls following normalization to actin. (E) Western blot analysis of β3 in cortex from individual 4 week old mice revealed dramatically reduced amounts of β3 in cre positive samples compared to cre negative control samples.