Loss of righting reflex (LORR) assay. A. Etomidate-induced LORR in pan-neuronal knockout mice was dependent on gender (p < 0.01). Therefore, male and female mice were analyzed separately. Both male and female pan-neuronal β3 knockouts were less sensitive to the sedative/hypnotic effects of etomidate (20 mg/kg) as evidenced by the reduced duration of the LORR compared to same sex controls. B. Ethanol-induced (3.5 g/kg) LORR did not differ between controls and pan-neuronal β3 knockouts. C. Etomidate-induced (20 mg/kg) LORR in forebrain selective β3 knockouts was not dependent on gender but knockouts were less sensitive compared to controls. D. Ethanol-induced (3.5 g/kg, i.p.) LORR of forebrain selective knockout mice did not differ compared to controls. *, p < 0.05; **, p ≤ 0.01.