Western blot analysis of pan-neuronal β3 knockouts. Western blot analysis of the β3 subunit of the GABAA-R from individual 7–9 week old mice revealed dramatically reduced amounts of β3 in hippocampus (A), cortex (B) and cerebellum (C) of Syn-cre positive samples compared to cre negative control samples. All blots were reprobed for β-actin as a loading control. Shown are representative western blots. Five mice of each genotype were analyzed and all samples were analyzed on at least two different blots. D. Summary graph of western blot analysis demonstrating a significant (*, p < 0.05;**, p < 0.01) reduction in β3 protein in all brain regions examined. Data are expressed as mean ± SEM of percent change in band intensity relative to cre negative controls following normalization to actin.