| Number of mice |  |  | |
---|---|---|---|---|
Experimental procedure(s) | KO | WT | Survival period | Analysis performed |
Transection of DC at T8; conditioning lesion of ipsilateral sciatic nerve; subsequent injection of CT-HRP into sciatic nerve for TGL | 8 | 6 | 21d | Regen growth by analysis of labelled sensory axons at/beyond DC lesion site |
Transection of dorsal spinal cord at C6; simultaneous injection of BDA into contralateral sensorimotor cortex | 6 | 6 | 21d | Regen growth by analysis of labelled CST axons at DC lesion site |
Transection of L5 dorsal root; conditioning lesion of ipsilateral sciatic nerve; subsequent injection of CT-HRP into sciatic nerve for TGL | 6 | 6 | 21d | Regen growth by analysis of labelled sensory axons in dorsal root and DREZ |
Sciatic nerve crush Sciatic nerve crush; saphenous nerve resection Sciatic nerve transection | 6 6 9 | 6 6 9 | n/a n/a n/a | Recovery of foot withdrawal (sensory) and toe spreading (motor) reflexes |
Sciatic nerve crush; subsequent injection of DiAsp into hindpaw skin | 10 | 10 | 5d (n = 2) 9d (n = 2) 13d (n = 8) 90d (n = 8) | Extent of sensory axon regen by analysis of retrogradely labelled DRG cells |
Sciatic nerve crush | 9 | 12 | 7d (n = 6) 10d (n = 15) | Extent of motor regen by analysis of silver-cholinesterase preparations of soleus muscle |
Sciatic nerve crush | 6 6 | 6 6 | 17d 21d (EM only) | Sensory reinnervation of skin by PGP 9.5 IHC; regen of axons into digital nerves by EM |
Sciatic nerve crush | 6 | 8 | 15d | Regen of axons into the distal nerve stump by EM |
Facial nerve crush: subsequent application of DiAsp to whisker pad | 4 | 4 | 14d | Extent of axon regen by analysis of retrogradely labelled facial neurons (n = 8) & recovery of whisker twitch and blink reflexes (n = 5) |
Facial nerve transection: subsequent application of FG or DiAsp to whisker pad | 12 | 12 | 30d (FG, n = 8) 40d (DiAsp, n = 4) | Extent of axon regen by analysis of retrogradely labelled facial neurons |