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Table 1 Utilisation of Animals

From: Analysis of axonal regeneration in the central and peripheral nervous systems of the NG2-deficient mouse

 

Number of mice

  

Experimental procedure(s)

KO

WT

Survival period

Analysis performed

Transection of DC at T8; conditioning lesion of ipsilateral sciatic nerve; subsequent injection of CT-HRP into sciatic nerve for TGL

8

6

21d

Regen growth by analysis of labelled sensory axons at/beyond DC lesion site

Transection of dorsal spinal cord at C6; simultaneous injection of BDA into contralateral sensorimotor cortex

6

6

21d

Regen growth by analysis of labelled CST axons at DC lesion site

Transection of L5 dorsal root; conditioning lesion of ipsilateral sciatic nerve; subsequent injection of CT-HRP into sciatic nerve for TGL

6

6

21d

Regen growth by analysis of labelled sensory axons in dorsal root and DREZ

Sciatic nerve crush

Sciatic nerve crush; saphenous nerve resection

Sciatic nerve transection

6

6

9

6

6

9

n/a

n/a

n/a

Recovery of foot withdrawal (sensory) and toe spreading (motor) reflexes

Sciatic nerve crush; subsequent injection of DiAsp into hindpaw skin

10

10

5d (n = 2)

9d (n = 2)

13d (n = 8)

90d (n = 8)

Extent of sensory axon regen by analysis of retrogradely labelled DRG cells

Sciatic nerve crush

9

12

7d (n = 6)

10d (n = 15)

Extent of motor regen by analysis of silver-cholinesterase preparations of soleus muscle

Sciatic nerve crush

6

6

6

6

17d

21d (EM only)

Sensory reinnervation of skin by PGP 9.5 IHC; regen of axons into digital nerves by EM

Sciatic nerve crush

6

8

15d

Regen of axons into the distal nerve stump by EM

Facial nerve crush: subsequent application of DiAsp to whisker pad

4

4

14d

Extent of axon regen by analysis of retrogradely labelled facial neurons (n = 8) & recovery of whisker twitch and blink reflexes (n = 5)

Facial nerve transection: subsequent application of FG or DiAsp to whisker pad

12

12

30d (FG, n = 8)

40d (DiAsp, n = 4)

Extent of axon regen by analysis of retrogradely labelled facial neurons

  1. KO = knockout; WT = wild-type; d = days post op; TGL = transganglionic labelling; CST = corticospinal tract; DC = dorsal column; DREZ = dorsal root entry zone; IHC = immunohistochemistry; EM = electron microscopy; FG = Fluorogold; Regen = regenerative/regeneration.