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Table 1 Utilisation of Animals

From: Analysis of axonal regeneration in the central and peripheral nervous systems of the NG2-deficient mouse

  Number of mice   
Experimental procedure(s) KO WT Survival period Analysis performed
Transection of DC at T8; conditioning lesion of ipsilateral sciatic nerve; subsequent injection of CT-HRP into sciatic nerve for TGL 8 6 21d Regen growth by analysis of labelled sensory axons at/beyond DC lesion site
Transection of dorsal spinal cord at C6; simultaneous injection of BDA into contralateral sensorimotor cortex 6 6 21d Regen growth by analysis of labelled CST axons at DC lesion site
Transection of L5 dorsal root; conditioning lesion of ipsilateral sciatic nerve; subsequent injection of CT-HRP into sciatic nerve for TGL 6 6 21d Regen growth by analysis of labelled sensory axons in dorsal root and DREZ
Sciatic nerve crush
Sciatic nerve crush; saphenous nerve resection
Sciatic nerve transection
6
6
9
6
6
9
n/a
n/a
n/a
Recovery of foot withdrawal (sensory) and toe spreading (motor) reflexes
Sciatic nerve crush; subsequent injection of DiAsp into hindpaw skin 10 10 5d (n = 2)
9d (n = 2)
13d (n = 8)
90d (n = 8)
Extent of sensory axon regen by analysis of retrogradely labelled DRG cells
Sciatic nerve crush 9 12 7d (n = 6)
10d (n = 15)
Extent of motor regen by analysis of silver-cholinesterase preparations of soleus muscle
Sciatic nerve crush 6
6
6
6
17d
21d (EM only)
Sensory reinnervation of skin by PGP 9.5 IHC; regen of axons into digital nerves by EM
Sciatic nerve crush 6 8 15d Regen of axons into the distal nerve stump by EM
Facial nerve crush: subsequent application of DiAsp to whisker pad 4 4 14d Extent of axon regen by analysis of retrogradely labelled facial neurons (n = 8) & recovery of whisker twitch and blink reflexes (n = 5)
Facial nerve transection: subsequent application of FG or DiAsp to whisker pad 12 12 30d (FG, n = 8)
40d (DiAsp, n = 4)
Extent of axon regen by analysis of retrogradely labelled facial neurons
  1. KO = knockout; WT = wild-type; d = days post op; TGL = transganglionic labelling; CST = corticospinal tract; DC = dorsal column; DREZ = dorsal root entry zone; IHC = immunohistochemistry; EM = electron microscopy; FG = Fluorogold; Regen = regenerative/regeneration.