Identification of novel proteins affected by rotenone in mitochondria of dopaminergic cells

BMC Neuroscience

Table 2 Summary of validation by WB and immunolocalization

Protein	IPI	Function	ASAP Ratio	WB of mitochondria	WB of cytosol	Co-localized with cyt C*
MAP2K2	00135708 00407256 00231331	Signaling	0	Low sensitivity	Low sensitivity	N/A
Sacsin	00124120 00373012	Folding degradation stability	2.26 ± 0.38	1.77 ± 0.09	0.82 ± 0.02	N/A
SNX1	00198369 00125441	Transport	2.21 ± 0.6	1.51 ± 0.03	Not detected	Yes
SHH	00123314	Signaling	5.47 ± 0.98	Low specificity	Low specificity	Yes
VAMP3	00132276 (00210971)	Transport	0.34 ± 0.07	0.48 ± 0.13	1.16 ± 0.15	N/A

*: Among the five candidate proteins, only antibodies against SHH and SNX1 demonstrated enough sensitivity and specificity for immunocytochemistry.

ISSN: 1471-2202