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Table 2 Summary of validation by WB and immunolocalization

From: Identification of novel proteins affected by rotenone in mitochondria of dopaminergic cells

Protein IPI Function ASAP Ratio WB of mitochondria WB of cytosol Co-localized with cyt C*
MAP2K2 00135708
00407256
00231331
Signaling 0 Low sensitivity Low sensitivity N/A
Sacsin 00124120
00373012
Folding degradation stability 2.26 ± 0.38 1.77 ± 0.09 0.82 ± 0.02 N/A
SNX1 00198369
00125441
Transport 2.21 ± 0.6 1.51 ± 0.03 Not detected Yes
SHH 00123314 Signaling 5.47 ± 0.98 Low specificity Low specificity Yes
VAMP3 00132276
(00210971)
Transport 0.34 ± 0.07 0.48 ± 0.13 1.16 ± 0.15 N/A
  1. *: Among the five candidate proteins, only antibodies against SHH and SNX1 demonstrated enough sensitivity and specificity for immunocytochemistry.