Functional classification of mitochondrial proteins with relative abundance changes after rotenone treatment. 110 proteins identified in the mitochondrial database MitoP2 and having 2-fold relative abundance changes after rotenone treatment were classified into the following categories: folding degradation stability, metabolism, morphology, respiratory chain, protein synthesis, signaling, transport, miscellaneous, and unknown functions. For a protein with multiple functions, it is assigned to the one that is best known. While this chart reflects the group as a whole, the distribution was the same regardless of response to rotenone. Functional classification of proteins for each of the four groups shown in Table 1 (ASAPRatio = 0, ≥ 2.0, ≤ 0.5, or = 999) resulted in distributions that were not different from each other (χ2 had P > 0.05 for the four groups).