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Figure 1 | BMC Neuroscience

Figure 1

From: Identification of novel proteins affected by rotenone in mitochondria of dopaminergic cells

Figure 1

Purity evaluation of isolated mitochondria by WB. Equal amounts of protein (20 μg for cytochrome C and nucleolin, 5 μg for tubulin) were loaded onto an 8–16% SDS-PAGE and analyzed by WB with indicated antibodies against marker proteins from mitochondria, cytosol, or nucleus. Antibodies against α-tubulin and nucleolin were used as markers for cytosolic and nuclear fractions, respectively. Antibodies directed against cytochrome C were used as markers for mitochondrial fraction.

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