Figure 5

Lack of rescue of Cisplatin induced neurite toxicity by PXD. Differentiated PC12 cells were incubated with Cisplatin alone or in combination with PXD for 24 hrs. The cells were washed and left for 24 hrs, then PXD at the concentrations indicated was added for a further 24 hrs before the percentage of cells with neurites was determined. The concentrations of PXD used in these experiments was 1 log lower than in the previous experiments. A) PXD alone had no effect on percent of cells with neurites. B) Cisplatin at 1 μg/ml showed no effect but at 20 μg/ml the cells failed to recover from the neurite toxicity (*p < 0.001 compared to control). PXD did not rescue the cells from the Cisplatin (20 μg/ml) induced neurite toxicity, although it showed 15% enhancement at 1 μM with 1 μg/ml Cisplatin (** p < 0.001). Data shows combined results of n = 3 experiments.