Effect of PXD Cisplatin on βIII-tubulin stained PC12 cells. Differentiated PC12 cells were incubated with Cisplatin alone or in combination with PXD for 24 hrs then fixed and immunostained for the neuronal marker βIII-tubulin. A) Control cultures showed a large percentage of cells with long neurites, which were also present in B) 100 nM PXD and C) 1 μM PXD. D) Significant cell and neurite toxicity was observed in cells incubated with 10 μM PXD. E) Cisplatin at 20 μg/ml decreased the percentage of cells bearing neurites compared to control, which was blocked by (F) 1 μM PXD. Scale bar in F represents 50 μm and applies to all panels in the figure.