Effect of Cisplatin and PXD on neurite length. Differentiated PC12 cells were incubated with Cisplatin alone or in combination with PXD for 24 hrs and the neurite length was determined by measuring the longest neurite on cells with neurites longer than 10 μm. A) PXD alone had no effect on neurite length up to a concentration of 1 μM, however 10 μM PXD decreased neurite length (#p < 0.001). B) Cisplatin at 1 μg/ml and at 20 μg/ml did not affect neurite length. PXD at 100 nM and 1 μM had little effect on neurite length in the presence of Cisplatin, although there was a slight increase in neurite length with PXD at 100 nM and 20 μg/ml Cisplatin (* p < 0.001). The combination of 10 μM PXD and Cisplatin at 1 μg/ml or 20 μg/ml was not different to the effect of 10 μM PXD alone. Data shows combined results of n = 3 experiments.