Hemocyte Location in Axon Guidance Mutants. In blue, RNA in situ hybridization showing expression for CG25C, a hemocyte probe. The CNS scaffold (brown) is stained with the BP102 antibody, and indicates the CNS phenotype. The ladder-like WT CNS is visible in WT; the collapsed phenotype is observed in slit, robo12 and slit.robo embryos. (3a), (3c), (3e) and (3g) are ventral views; (3b), (3d), (3g) and (3h) are lateral views. Green arrows indicates the middle segments where in some mutants the hemocytes are absent. (3a) and (3b) are WT embryos where the hemocytes are present ventrally along the entire anterior-posterior axis. (3c) and (3d) are homozygous slit embryos where the hemocytes are not present in the middle section of the embryo. (3e) and (3f) are double mutants for robo and robo2 (robo12). The collapsed midline phenotype is similar to slit and slit.robo mutants, and yet, contrary to slit and slit.robo, the hemocytes are present throughout the whole embryo. (3g) and (3h) are embryos double mutant for robo and slit. They are similar to the slit homozygous mutants in that the hemocytes are not present in the middle section of the embryo.