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Figure 5 | BMC Neuroscience

Figure 5

From: Calcium signals can freely cross the nuclear envelope in hippocampal neurons: somatic calcium increases generate nuclear calcium transients

Figure 5

Photolysis-induced cytosolic calcium elevations are followed by increases in nuclear calcium concentrations. A, schematic illustration of the position of the 5 μm2 area (pink circle) in the cytosol that was illuminated with UV light for photolysis of NP-EGTA; arrows indicate the directions of calcium signal spreading analyzed. B, the positioning of the uncaging spot was guided by MitoTracker staining shown in red. C, time series of raw Fluo-4 fluorescence images of the calcium measurements shown in D and E; the indicated times correspond to the time scale of the graphs. The 5 μm2 areas in the cytoplasm that were illuminated with UV light for photolysis of NP-EGTA are indicated with white circles. D and E, time courses of calcium transients are shown at distances of 6 μm and 10 μm from the uncaging spot in the nucleus (continuous black line), in the cytoplasm (dotted red line), and at the perinuclear space (dashed green line); arrows point towards the positions of measurements (filled white squares). Images were taken every 401 msec. UV light was switched on as indicated by the dashed black line (t exp = 10.2 msec). Scale bar is 5 μm.

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