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Table 2 Region- or state-related differences in activation of ERK2 in naïve and pain-experiencing rats.

From: Region- or state-related differences in expression and activation of extracellular signal-regulated kinases (ERKs) in naïve and pain-experiencing rats

 

Spinal cord dorsal horn

S1 area of cortex

Hippocampus

 

Naïve

Saline

Bee venom

Naïve

Saline

Bee venom

Naïve

Saline

Bee venom

5 min

         

   Ipsil

0.01 ± 0.01

1.40 ± 0.74*

1.32 ± 0.51†

0.43 ± 0.08

1.03 ± 0.31

0.85 ± 0.18

0.53 ± 0.18

0.61 ± 0.29

0.81 ± 0.13

   Contl

0.02 ± 5.12 × 10-3

1.80 ± 0.36**

1.93 ± 0.36††

0.36 ± 0.05

0.86 ± 0.20

0.79 ± 0.18

0.58 ± 0.18

0.52 ± 0.23

0.77 ± 0.31

15 min

         

   Ipsil

9.51 × 10-3 ± 3.94 × 10-3

1.21 ± 0.40*

0.56 ± 0.15†

0.37 ± 0.10

0.91 ± 0.17

0.64 ± 0.22

0.55 ± 0.21

0.70 ± 0.10

0.67 ± 0.24

   Contl

0.01 ± 7.74 × 10-3

1.50 ± 0.31**

1.31 ± 0.28††

0.37 ± 0.04

0.75 ± 0.20

0.79 ± 0.15

0.49 ± 0.14

0.62 ± 0.18

0.71 ± 0.16

30 min

         

   Ipsil

6.09 × 10-3 ± 1.20 × 10-3

0.93 ± 0.29

1.08 ± 0.40†

0.25 ± 0.10

0.72 ± 0.22

0.59 ± 0.20

0.49 ± 0.19

0.64 ± 0.27

0.79 ± 0.25

   Contl

0.03 ± 0.02

1.45 ± 0.30*

1.34 ± 0.32†

0.26 ± 0.06

0.78 ± 0.22**

0.81 ± 0.12††

0.46 ± 0.17

0.52 ± 0.06

0.69 ± 0.07

1 h

         

   Ipsil

0.01 ± 7.56 × 10-3

1.16 ± 0.46*

1.00 ± 0.30†

0.16 ± 0.08

0.89 ± 0.45

0.91 ± 0.33

0.38 ± 0.17

1.04 ± 0.34

1.22 ± 0.27

   Contl

0.06 ± 0.04

1.33 ± 0.20**

1.26 ± 0.20††

0.15 ± 0.07

0.67 ± 0.27

0.55 ± 0.22

0.44 ± 0.15

1.13 ± 0.40

5.29 ± 4.20

2 h

         

   Ipsil

9.31 × 10-3 ± 2.83 × 10-3

1.35 ± 0.16*

1.21 ± 0.41†

0.22 ± 0.11

0.62 ± 0.15*

0.52 ± 0.04†

0.47 ± 0.19

1.08 ± 0.12*

1.43 ± 0.17††

   Contl

0.03 ± 0.02

1.21 ± 0.19*

1.52 ± 0.40††

0.22 ± 0.10

0.53 ± 0.12*

0.65 ± 0.11†

0.46 ± 0.17

1.19 ± 0.27

1.14 ± 0.31

4 h

         

   Ipsil

0.03 ± 0.03

0.69 ± 0.19*

0.76 ± 0.25†

0.25 ± 0.07

0.73 ± 0.17*

0.77 ± 0.14†

0.61 ± 0.31

0.94 ± 0.28

1.38 ± 0.13

   Contl

0.02 ± 9.34 × 10-3

1.11 ± 0.12**

1.40 ± 0.33††

0.40 ± 0.05

0.93 ± 0.23*

1.10 ± 0.23†

0.55 ± 0.16

0.74 ± 0.05

1.00 ± 0.16

6 h

         

   Ipsil

0.03 ± 0.01

0.67 ± 0.16**

0.63 ± 0.14††

0.34 ± 0.08

1.19 ± 0.44

0.78 ± 0.13

0.75 ± 0.18

1.38 ± 0.36

1.52 ± 0.27

   Contl

0.02 ± 6.72 × 10-3

1.20 ± 0.09***

1.19 ± 0.14†††

0.32 ± 0.05

1.34 ± 0.63

0.93 ± 0.18

0.67 ± 0.17

1.85 ± 0.57

1.49 ± 0.20

12 h

         

   Ipsil

3.31 × 10-3 ± 1.67 × 10-3

0.75 ± 0.18**

0.73 ± 0.09††

0.26 ± 0.04

0.82 ± 0.24

0.87 ± 0.20

0.55 ± 0.24

1.15 ± 0.27

1.27 ± 0.14

   Contl

0.04 ± 5.41 × 10-3

1.38 ± 0.19**

1.58 ± 0.42††

0.19 ± 0.05

1.43 ± 0.63

1.64 ± 0.58

0.64 ± 0.17

2.11 ± 0.89

1.63 ± 0.14

24 h

         

   Ipsil

2.81 × 10-3 ± 7.82 × 10-4

0.81 ± 0.16**

0.79 ± 0.15††

0.11 ± 0.04

1.12 ± 0.59

2.07 ± 1.45

0.44 ± 0.17

1.13 ± 0.40

1.00 ± 0.28

   Contl

0.01 ± 7.74 × 10-3

1.58 ± 0.34*

1.06 ± 0.15†

0.09 ± 0.03

1.75 ± 0.47

2.22 ± 1.74

0.61 ± 0.33

2.77 ± 0.93

1.24 ± 0.23

48 h

         

   Ipsil

3.65 × 10-3 ± 2.52 × 10-3

0.74 ± 0.26

0.90 ± 0.34†

0.18 ± 0.06

0.56 ± 0.23

0.48 ± 0.15

0.56 ± 0.27

1.22 ± 0.18

0.76 ± 0.08

   Contl

0.08 ± 0.07

1.63 ± 0.21*

2.07 ± 0.66†

0.07 ± 0.01

0.65 ± 0.38

0.73 ± 0.36

0.70 ± 0.38

1.22 ± 0.09

0.86 ± 0.27

  1. Notes: Data are mean ± SEM. for the immunoblotted band intensities of phospho-ERK2 normalized to those of the loading control (total ERK2) obtained from three rats per group. Values from ipsilateral (Ipsil.) and contralateral (Contl.) sides of three indicated areas at ten time points are shown. * P < 0.05, ** P < 0.01, *** P < 0.001, saline-treated rats versus naïve control. † P < 0.05, †† P < 0.01, ††† P < 0.001, bee venom-treated rats versus naïve control.