Nerve fibers and ganglia in porcine pancreas double immunostained for CART (A, D, G, J) and VIP (B, E, H, K); merged in C, F, I, L. A–C: Large nerve trunk. D–F: delicate nerve fiber. G–I: Intrapancreatic ganglion. Note high degree of colocalisation of CART and VIP in nerve fibers and nerve cell bodies. J–L: CART IR nerve cell body devoid of VIP, but surrounded by VIP IR fibers. Colocalisation exemplified with arrowheads. Scale bars = 20 μm.