Figure 4

VP22-mediated microdystrophin gene expression was more efficient in reducing central nucleation in mdx mice. Histological analysis the left TA of mdx mice injected by pAMICDYS, pAVP22-MICDYS and the right TA injected by PBS. Shown are H&E-stained TA muscle cross sections from post-injection 2 weeks (A, B, C), post-injection 16 weeks (D, E, F). The left TA muscles treated by pAMICDYS (B, E), pAVP22-MICDYS (C, F); the right TA muscles treated by PBS (A, D). Magnification: × 200. Centrally nucleated fibers were counted in transverse H&E cryosections from treated mdx mice. The ratio of centrally nucleated fibers (%) is shown as means ± SE in TA muscle at post-injection 2 weeks (G) and 16 weeks (H). * p < 0.05 compared with mdx mice; ⁺p < 0.05 compared with pAMICDYS transfered mice.