TRPM5-expressing cells of the gastrointestinal tract have morphological and biochemical characteristics of brush cells. (A-D) Morphological features of TRPM5-expressing cells. Confocal images of TRPM5-specific signals (left panels) and overlay with corresponding DIC images (right panels) are shown. The TRPM5-enriched cells in gastric glands of mouse stomach (A, B) and in mouse a duodenal villus (C, D) reveal a long basolateral processes (indicated by the arrows). Within duodenal epithelia (C, D), the TRPM5-immunoreactive cell contains a protrusion extending beyond the epithelial luminal border (indicated by an arrowhead). (E-G) Co-localization of TRPM5 with CK18 in intestinal glands of the rat duodenum. Confocal images of TRPM5 and CK18 specific signals (E, F) and their overlay with corresponding DIC images (G) are depicted. Scale bars are 10 μm.