Characterization of growth, morphology and nestin in undifferentiated cells in culture. Cell lines are confirmed to express the myc transgene as shown by RT-PCR (A). Images of ReNcell VM (B) and CX (C) as monolayer cultures. D) Growth of cells within a passage showing cells plated at ~5000 cells/cm2 achieve confluency within 3 days in culture with equivalent growth rates as measured using CyQuant fluorescent assay. ReNcell lines are positive for the neural stem cell marker nestin by western blot (E) and immunocytochemistry in green on ReNcell VM (F) and CX (G) with Hoechst nuclear counter stain in blue. (B, C) and (F, G) compare the two cell lines, scale bar: 100 μm.