Figure 2

GLU changes are presented as mean percent change from the median of four baseline collections (± SEM) for the dialysates indicated on the abscissa. A combination of 250 μM-AA CSF and "Lights ON" conditions were utilized wherever white or grey-filled icons appear. Black-filled icons indicate when animals were either responding to darkness (A & B), treated with 1000 μM AA (C & D), or both (B & D). The within-subjects factor was AA-treatment, as all animals were treated with both AA levels, 250 μM and 1000 μM AA, in separate dialysis sessions during the experimental period indicated below each graph. Separate graphs depict the GLU level changes occurring in response to lights-out (A & B), or AA treatment (C & D). The boxes within each graph depict time points where separate analyses were performed (last baseline through 45 min dashed, last baseline through 75 min solid). Repeated measures ANOVA analyses within the dashed boxes were significant in A, B, & C. The first 45-min was the only period during which a significant GLU increases in response to AA treatment across collections was exposed, and only during the "LIGHTS ON" experiment (C). Note the large effects of darkness exposure (Experimental period, black-filled icons) on striatal GLU levels depicted in graphs A & B. When the two "Post" collections (black & grey-filled versus white-filled) were included, significant increases in GLU levels occurred across all collections (15–120 min) compared to baseline when animals treated with 1000 μM AA were exposed to darkness (B). However increases in GLU were only significant during the Experimental period (15–75 min) when animals were treated with 250 μM AA (A).