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Figure 6 | BMC Neuroscience

Figure 6

From: Human immunodeficiency virus type 1 efficiently binds to human fetal astrocytes and induces neuroinflammatory responses independent of infection

Figure 6

Induction of cytokines by exposure of HFA to HIV-1. A-C) HFA were mock-infected (panel A); infected with VSV/NL4-3, a VSV-G pseudotyped NL4-3 incapable of expressing gp120 (panel B), or infected with NL4-3 (panel C), washed, and after 24 h incubation cell supernatants were collected and applied to RayBio Human Cytokine Antibody Array 3.1. The map of antibodies present on the array can be accessed at http://www.raybiotech.com/map/human_III_map.pdf. D) HFA were infected as indicated, washed, and after 24 h incubation supernatants were collected for assay of IL-6 levels by ELISA. E) The expression of IL-6 (upper panel) and IL-8 (lower panel) mRNA by HFA was measured by qPCR at the indicated times after mock infection or NL4-3 infection.

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BMC Neuroscience

ISSN: 1471-2202

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