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Figure 4 | BMC Neuroscience

Figure 4

From: Human immunodeficiency virus type 1 efficiently binds to human fetal astrocytes and induces neuroinflammatory responses independent of infection

Figure 4

Dynamics of GFP-Vpr/HIV-1 association with HFA or MAGI cells at 37°C. MAGI cells were exposed to NL4-3 carrying GFP-Vpr (first row) or to the fusion incompetent HIV-1/517B carrying GFP-Vpr (second row) or HFA were exposed to NL4-3-GFP-Vpr. Cells were incubated with virus at 37°C and fixed at 1 h (first column), 6 h (second column), or 18 h (third column) after infection. HeLa cells were stained with anti-actin and HFA were stained with anti-GFAP, with DAPI and with Texas Red-labeled secondary antibodies. Images shown are superimpositions of red and green fluorescence.

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