Figure 2From: A novel three-dimensional system to study interactions between endothelial cells and neural cells of the developing central nervous systemBrain EC migration into neurospheres. Mouse brain EC were prepared, labelled with cell tracker, and then plated onto mouse neurospheres, as described in Methods. 5 days later, the distribution of fluorescent-labelled EC within neurospheres was examined by fluorescent microscopy of frozen sections of EC-neurosphere co-cultures, using Hoechst (blue) to identify all cell nuclei (A and C) and the FITC channel (green) to identify the cell tracker-labelled EC (B and D). Scale bar = 100 μm. Panel E shows a histogram of the distribution of EC number within neurospheres in a representative experiment (n = 3). Note that EC were detected in the majority of neurospheres.Back to article page